Monitoring genetic transformation with RUBY visible reporter in Nicotiana tabaccum L. ($) https://botany.fyi/6fxs13
Jogam et al transformed Nicotiana tabacum with a RUBY vector construct consisting of three gene clusters, CYP76AD1, DODA, and 5-GT, along with a hygromycin selective marker for visible reporter study. After three weeks of transformation, the betalain pigments were easily detected in the explant leaf tissue.
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