bblkargonaut

@bblkargonaut@lemmy.world

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bblkargonaut ,

A partial solution is to remove nimby laws that prevent accessory dwelling units, duplexes, and medium and high density housing. Then you use the tax laws to heavily punish corporate ownership of low and medium density housing, and make it progressively more expensive for mom and pop landlords after a reasonable number of properties.

Hopefully this would lower the cost for single family housing, while increasing supply and variety of rentals. But somehow you'd have to prevent end stage capitalist collusion from fixing prices so competition could actually work to drive down prices.

bblkargonaut ,

Pretty good description. Few extra points.
-you don't need to don't need to do PCR to have enough Nucleic acids to visualize on the gel. Often you use gels to visualize things like purified plasmids. You can also digest your plasmids with enzymes to confirm genomic insert.
-these type of gels have many uses outside of direct comparisons, for example you can use the gel purity DNA of a particular size by cutting out the band, melting the gel and using a silica column to pull the DNA out of the melted gel slice. This is super common when you do molecular cloning.

For what is possibly wrong with the top gel.

  • the wavy line in some bands indicates uneven current, this can be due to sample composition before loading the gel, like having cellular debris along with your DNA or a mismatch between the buffer used to make the gel and the running buffer.

-the blurred bands can be a lot of things. It might just be inherited to the sample type, for example raw genomic DNA will look like this because you'll have enzyme that degrade DNA, and a lot of mid replication chromosomes resulting in fragments existing across a spectrum of sizes equally. Additionally the it could also be user error with overloaded well or not enough loading buffer in the sample so it's not pushed in to gel evenly, or it was run to fast.

Overall the top gel just looks like crap and the bottom gel looks great, but depending on what was run you really don't know what the gel should look like, if I was testing endonuclease activity the top gel would probably be what I was hoping to get. If was doing PCR on single gene, the bottom one is better.

bblkargonaut ,

I'm a noob, I started 2 months ago with immich and tailscale. Now I have an unraid server, it's a slippery slope.

bblkargonaut ,

Lol yeh but it's partially funded by money saved from canceled subscriptions.

bblkargonaut ,

I really hope we get the last few expanse books as a movie trilogy with the TV series actors in like 5 years so the actors can age appropriately for the time gap accounting for anti aging meds. Maybe ignore TV series actual ending and recast certain actors so we can get some of the best parts of the last few books.

bblkargonaut ,

Netflix has been such a staple for so long it took a full Arr suite to get my wife to be ok that I cancelled it. When it came to Disney plus, she didn't care because she's not into Star wars and she hulk soured her on marvel. My 5 year old niece and nephew only ask to watch Netflix when they come over. As someone who has witnessed them kill so many franchises I love, I think Disney cracking down on password sharing is a great idea.

bblkargonaut ,

I don't think the general public appreciates how fing weird bird genomics are, and how this was basically impossible without machine learning.

bblkargonaut ,

My family owns a few rental properties. It was the mechanism that allowed my grandfather who grew up in 1930s Mississippi working the same land his slave grandparents did to escape poverty and retire pretty well off. He moved to Chicago, worked as a garbage man, bought a 3 flat and lived in one unit and rented the other two. Eventually he invested his money in more properties and and had his lawyer buy his house in the racist suburb of Oak Park so my mother could grow up in relative comfort.

The company was never ment to extract unlimited profit, we actually had many unprofitable years due to demographics changes, recessions, maintenance, and poor tenants causing damage ( who flushes weave down the drain?). But in aggregate it's made enough to give stability, because being a landlord is always our side job.

During the pandemic my mother worked with all the tenants who lost their jobs or had limited or no income. Since none of our properties have a mortgage, she reduced rent to just enough to cover the insane Illinois property taxes and the shared utilities for the people that could pay. When the boiler went out she picked up a few extra nursing shifts with covid pay to cover it. When things returned to the new normal or when tenants found new work, she just had them resume normal rent without needing to pay any back rent in their lease.

You'd think we would be rewarded for doing the right thing and treating people with basic human decency, but no. When we applied for covid assistance, the money was gone. We then started to receive building violations for one of our properties is an up and coming area. The funny thing about these violations was that they were for items repairs 10 years earlier, and also for things we received city and used city grants and contracts to fix. Now we are currently in a legal battle with the city where they want us to take a $900k loan to fully renovated the building or have the city seize the property because it's a "crime den" in their words. Like how, we screen everyone, rent mostly to old people and single mothers, and have camera in public areas and around the buildings. We even routinely provide footage to the police when they request it, even though that means we have to buy a new DVR since we have yet to have one returned.

But ever since covid and this inspection bull, we get daily calls letters, emails from corporations expressing their interest in buying our not for sale property.

bblkargonaut ,

Exactly

bblkargonaut ,

The funny thing is that we have to be present during inspections because we have to allow them into utility areas and different units along with giving tenants notice that people will enter into their homes. We passed 2019's inspection and was working on getting leed grant to help finish some improvement needed to qualify to accept section 8 renters since we just replaced all the windows and installed central air conditioning in each unit. The violations were for things corrected across almost 20 years of inspections. There was no inspection that led to our violations.

bblkargonaut ,

Sweet summer child

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